Department of Pharmaceutical Biochemistry

Division Environmental and Sociomedical Sciences
Great Department Environmental and Analytical Sciences
Website http://square.umin.ac.jp/yseika/engsite/engindex.html
Staff
Professor Shozo Shoji
shoji@gpo.kumamoto-u.ac.jp
Associate professor Shogo Misumi
misumi@gpo.kumamoto-u.ac.jp
Instructor Nobutoki Takamune
tkmnnbtk@gpo.kumamoto-u.ac.jp
Research theme


[Full size image]

A (Arrow head) Proteome Analysis showed that cyclophilin A located outside the virion.
(J. Virol. 76,10000-10008. (2002))
At the end of 2002, the number of HIV infections was estimated at about 42 million. To develop novel AIDS vaccines and anti-HIV agents, the following subjects are being undertaken In our research field.
  1. Biochemical studies of HIV-1 coreceptor-based vaccine
  2. Molecular biological studies of protein N-myristoylation
  3. Proteome studies of HIV-1
  4. Molecular biological studies of HIV-1 protease suicide inhibition
  5. Molecular biological studies of HIV-1 Tat
  6. Basic studies of development of anti-HIV-1 chemotherapeutic drug
[Recent publications]
  1. Shogo Misumi, Reina Nakajima, Nobuoki Takamune, and Shozo Shoji. A cyclic dodecapeptide-multiple-antigen-peptide-conjugate from undecapeptidyl arch (from Arg168 to Cys178) of extracellular loop2 in CCR5 as a novel HIV-1 vaccine. J. Virol. 75: 11614-11620. 2001
  2. Misumi, S., Fuchigami, T., Takamune, N., Takahashi, I., Takama, M., and S.Shoji. Three isoforms of cyclophilin A associated with human immunodeficiency virus type 1 were found by proteomics by using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Virol. 76: 10000-10008. 2002
  3. Takamune, N., Hamada, H., Sugawara, H., Misumi, S., and S. Shoji, S. Development of an enzyme-linked immunosorbent assay for measurement of activity of myristoyl-coenzyme A:protein N-myristoyltransferase. Anal Biochem. 309: 137-142. 2002
  4. Takamune, N., Hamada, H., Misumi, S., and S. Shoji. Novel strategy for anti-HIV-1 action: selective cytotoxic effect of N-myristoyltransferase inhibitor on HIV-1-infected cells. FEBS Lett. 527: 138-142. 2002
  5. Fuchigami, T., Misumi, S., Takamune, N., Takahashi, I., Takama, M., and S. Shoj. Acid-labile formylation of amino terminal proline of human immunodeficiency virus type 1 p24gag was found by proteomics using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Biochem. Biophys.Res.Commun. 293: 1107-1113. 2002
  6. Ando, Y., Nakamura, M., Kai, H., Katsuragi, S., Terazaki, H., Nozawa, T., Okuda, T., Misumi, S., Matsunaga, N., Hata, K., Tajiri, T., Shoji, S., Yamashita, T., Haraoka, K., Obayashi, K., Matsumoto, K., Ando, M., and M. Uchino. A novel localized amyloidosis associated with lactoferrin in the cornea. Lab Invest. 82:757-766. 2002
  7. Haraoka, K., Ando, Y., Ando, E., Sun, X., Nakamura, M., Terazaki, H., Misumi, S., Tanoue, Y., Tajiri, T., Shoji, S., Ishizaki, T., Okabe, H., and H.Tanihara. Presence of variant transthyretin in aqueous humor of a patient with familial amyloidotic polyneuropathy after liver transplantation. Amyloid, 9: 247-251. 2002
  8. Ando, Y., Terazaki, H., Haraoka, K., Tajiri, T., Nakamura, M., Obayashi, K., Misumi, S., Shoji, S., Hata, K., Nakagawa, K., Ishizaki, T., Uemoto, S., Inomata, Y., Tanaka, K., and H. Okabe. Presence of autoantibody against ATTR Val30Met after sequential liver transplantation. Transplantation. 73: 751-755. 2002
  9. Misumi ,S., Endo, M., Mukai, R., Tachibana, K., Umeda, M., Honda, T., Takamune, N., Shoji, S. A novel cyclic peptide immunization strategy for preventing HIV-1/AIDS infection and progression. J. Biol. Chem. 278:32335-32343. 2003
  10. Misumi,S., Takamune,N., and Shoji, S. Proteomics of HIV-1 virion. BIOMEDICAL AND PHARMACEUTICAL APPLICATIONS OF PROTEOMICS (Editor H. Hondermarck) Kluwer Academic Publisher, The Netherland. Chapter 14 2003.
  11. Misumi,S., Takamune,N., Ohtsubo,Y., Waniguchi,K., and Shoji,S. Zn2+ binding to cysteine-rich domain of extracellular human Immunodeficiency virus type-1 Tat protein is associated with Tat protein-induced apoptosis. AIDS Res. Hum. Retroviruses. in press